Proteomics

 

The Radboud Proteomics Center embodies the core expertise on proteomics and its applications in biomedical research, being one of the Technology Platforms within the Radboud UMC. Using a considerable variety of mass spectrometry platforms, proteomics applications range from straightforward identification of proteins and their modifications, to comprehensive profiling of biological system and de novo mapping of yet unknown (sub)proteomes. Special focus resides in the study of protein complexes that are related to biomedical function (including their composition, modifications and regulation) and considerable expertise has been build in the past years on mitochondrial complexes. 

The interaction of the Radboud Proteomics Center with the departemental metabolomics, glycomics and diagnostic laboratories enables a smooth translation of findings to implementation in clinical care, one of the other focus points of the proteomics laboratory.

Recent publications

  1. Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling. Wessels HJ, Vogel RO, Lightowlers RN, Spelbrink JN, Rodenburg RJ, van den Heuvel LP, van Gool AJ, Gloerich J, Smeitink JA, Nijtmans LG. PLoS One. 2013 Jul 23;8(7):e68340.

  2. A comprehensive full factorial LC-MS/MS proteomics benchmark data set. Wessels HJ, Bloemberg TG, van Dael M, Wehrens R, Buydens LM, van den Heuvel LP, Gloerich J. Proteomics. 2012 Aug;12(14):2276-81.

  3. Pinpointing biomarkers in proteomic LC/MS data by moving-window discriminant analysis. Bloemberg TG, Wessels HJ, van Dael M, Gloerich J, van den Heuvel LP, Buydens LM, Wehrens R. Anal Chem. 2011 Jul 1;83(13):5197-206.

  4. Liquid chromatography-mass spectrometry-based proteomics of Nitrosomonas. Wessels HJ, Gloerich J, van der Biezen E, Jetten MS, Kartal B. Methods Enzymol. 2011;486:465-82.

  5. Automated measurement of permethylated serum N-glycans by MALDI-linear ion trap mass spectrometry. Guillard M, Gloerich J, Wessels HJ, Morava E, Wevers RA, Lefeber DJ. Carbohydr Res. 2009 Aug 17;344(12):1550-7.