Structure and Function of Mitochondrial Complexes
Proteins are the molecules that form the structural basis and execute the vast majority of functions in all cells. Elucidating their structure and function and understanding their interactions and dynamics therefore forms the indispensable basis for achieving profound knowledge about physiology of an organism and to develop causal treatment strategies of human disease. In our quest to gain insight into the molecular basis of mitochondrial disease we currently focus on two topics:
1. Atomic structure and mechanism of mitochondrial complex I
We have elucidated the structure of mitochondrial complex I by X‐ray crystallography and aim at understanding its molecular mechanism by detailed functional analysis of site-directed mutants in the yeast genetic model Yarrowia lipolytica with respect to redox-driven proton translocation, control of the active/deactive transition and ROS formation. We use the information obtained by these studies to reach a detailed understanding of the pathophysiological mechanisms of human mutations causing mitochondrial disorders. To this end, complementary studies are performed in human (patient) cell lines.
2. Mitochondrial complexomics
Many mitochondrial functions are linked to the stable or transient formation of multiprotein complexes. For a comprehensive analysis of the inventory of such complexes in a given biological sample, we have developed complexome profiling. This approach links state of the art shotgun proteomics to the superior separation power of blue-native electrophoresis for native protein complexes. Protein-migration profiles of complexes of up to a size of 30 MDa are obtained that are then analyzed by bottom-up hierarchical clustering for the unbiased detection of their constituents. Complexome profiling allows for the discovery of functionally important components of macromolecular assemblies and assembly inter-mediates. Time course dependent complexome profiling provides comprehensive insights into the functional dynamics of protein-protein interactions. Our current goals are to obtain detailed insight into the assembly dynamics of mitochondrial complexes in health and disease and to decipher the composition and functional dynamics of the protein complexes associated with mitochondrial permeability transition.